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KMID : 0545120020120040706
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 4 p.706 ~ p.709
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Construction and Characterization of a Recombinant bioluminescence Streptomycetes for Potential Environmental Monitoring
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PARK, HYUN-JOO
HWANG, KEUM-OK/KIM, EUNG-SOO
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Abstract
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Bacterial bioluminescence has been known to be a highly valuable reporter system for its potential application as an effective and simple environmental monitoring method for toxic compounds. In this short report, we constructed a streptomycetes-Escherichia coli shuttle vector-containing bioluminescence system and evaluated its potential application for toxic compounds monitoring. The luxAB bioluminescence genes from Vibrio harveyi were cloned into a streptomycetesE. coli shuttle vector (named pESK004) and functionally expressed in Streptomyces lividans. The recombinant S. lividans containing pESK004 exhibited an optimal bioluminescence at the optical density (OD_600㎚) of 0.4-0.5 and aldehyde concentration of 0.005%. When the recombinant bioluminescence streptomycetes was exposed to a toxic compound such as heavy metals, chlorinated phenols, or pesticides, the bioluminescence was decreased proportionally to the concentration of toxic compound in the assay mixture. The EC_50 (effective concentration to decrease 50% of the bioluminescence prior to exposure) values in the recombinant bioluminescence streptomycetes for mercury, 2,4-dichlorophenol, and malathion were measured at 2.2 ppm, 144.0 ppm, and 82.4 ppm, respectively. The degree of sensitivity and specificity pattern toward these toxic compounds characterized in this recombinant bioluminescence streptomycetes were unique when compared with previously reported bacterial bioluminescence systems, and this revealed that a recombinant bioluminescence streptomycetes might provide an alternative or complementary system for potential environmental monitoring.
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